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KMID : 1164219900040010071
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Journal of Korean Society for Radiotherapeutic Technology
1990 Volume.4 No. 1 p.71 ~ p.77
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Effect of ionizing radiation on cultured submandibular gland (SG) cells of mouse
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Lee Song-Jae
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Abstract
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The present study was undertaken to investigate effects of ionizing radiation on DNA synthesis and chromosomal abnormality in cultured submandibular gland(SG) cells. SG cells from C57BL/6N Crj mice were cultured in Dulbecco’s modified Eagle’s medium (DME) supplemented with fetal bovine serum, antibiotics and fungizone. The cultured SG cells were irradiated with graded doses of gamma ray () at a dose rate of 58.4rad/min. The effect of irradiation of on DNA synthesis in cultured cells was evaluated by measuring the incorporation of 3H-TdR. Using conventional chromosome techniques and Giemsa staining methods, chromosomal abnormalities in cultured SG cells, induced by irradiation of werw examined. Cytological observations were carried out by a light microscope with high resolving power. The results obtained were as follows : 1. DNA synthesis of SG cells was quantitatively dependent on a radiation dose compare to control. 2. A polyploids and few chromosome-type break, such as single and double breaks, deltions and triradial figures were more predominantly in irradiated SG cells than in control. This increase of chromosomal abnormality was in the proposition to the irradiation doses.
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KEYWORD
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